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Image Search Results
Journal: Pediatric nephrology (Berlin, Germany)
Article Title: Glomerular endothelial cells and podocytes can express CD80 in patients with minimal change disease during relapse
doi: 10.1007/s00467-020-04541-3
Figure Lengend Snippet: (A) Immunostaining with a mouse monoclonal anti human CD80 (red) was performed on paraffin embedded sections from patients with MCD and FSGS. Positive and negative controls are shown (upper panel). Upregulation of glomerular CD80 was noted in most of MCD but few FSGS patients (lower panel). (B) CD80 (green, upper panel) colocalized with caveolin 1 in an endothelial specific pattern (arrows). To less extent, CD80 (red, lower panel) colocalized with nephrin (arrowheads). (C) Specificity of immunostaining was confirmed by silver staining. No signal detected in kidney tissue sample from same MCD patient in relapse when primary antibody was omitted. (D) Electron microscopy (EM) from kidney tissue from patient with MCD is shown. CD80 colocalized with glomerular endothelial cells and rarely podocytes. Original EM images captured at 6000x (far right) and 30000x. Podocyte (P), capillary lumen (CL), endothelial cell (EC), urinary space (U), glomerular basement membrane (GBM). Scale bars: 100 μm.
Article Snippet: Reagents and antibodies The following primary antibodies were used CD80 (AF740, R&D) at 1:100 dilution; CD80 (MAB140, clone 37711, R&D) at 1:20; caveolin 1 (D46G3, #3267, Cell Signaling) at 1:800,
Techniques: Immunostaining, Silver Staining, Electron Microscopy